Spesifikasi:
Scanner: Two independent, galvanometric scanning mirrors with ultrashort line and frame flyback
Scanning resolution: 32 × 1 to 6,144 × 6,144 pixels (Airyscan 2 max. 4,096 × 4,096 pixels), also for multiple channels, continuously adjustable (for each axis)
Scanning speed:
At 512 × 512 pixels: confocal – up to 8 fps; Airyscan SR – up to 4 fps; Multiplex SR-2Y – 8.4 fps; Multiplex SR-4Y – 18.9 fps
At 512 × 64 pixels: confocal – up to 64 fps
Scanning zoom: 0.45 × to 40 ×; continuously adjustable
Scanning rotation: Can be rotated freely (360°), adjustable in increments of 0.1°, freely adjustable xy offset
Scanning field: 20 mm diagonal in the intermediate image plane, with full pupil illumination
Pinhole: Master pinhole with preset size and position; can be adjusted as desired for multitracking and short wavelengths
(such as 405 nm); automatic alignment
Beam path: One major beam splitter for four laser lines (405, 488, 561 and 640 nm) at 10 degree with excellent laser line suppression. The 640 nm laser line can be
used for internal autofocusing. Depending on the system, either one or two patented Variable Secondary Dichroics (VSD) can
be used to flexibly divert the respective spectral range of light to chosen channels. Emission filters can be used to clean up the signal when imaging
autofluorescent or highly scattering samples
Physical Dimensions Length (cm) Width (cm) Height (cm) Weight (kg):
Small actively and passively damped system table: 90 - 75 - 83 - 130
Large actively damped system table (incl. corner pieces): 120 (129) - 90 (99) - 87 - 180
Vibraplate for Axio Imager (consists of three pedestals): 32 - 30 - 4.5 - 1.5
Detectors:
2 spectral detection channels, GaAsP (typical QE 45 %) or multialkali (MA) PMT (typical QE 25 %); LSM Plus: resolution down to 160* nm lateral,
500 nm axial with pinhole at 0.8 AU; resolution down to 120* nm lateral, 500 nm axial with pinhole at 0.3 AU
1 additional GaAsP PMT, MA PMT or Airyscan 2 detector
Airyscan 2 for spatial detection (GaAsP) with 40×, or 63×, or 100× objectives; for super-resolution (down to 120* nm lateral, 350 nm axial;
with jDCV 90* nm lateral, 270 nm axial) or Multiplex acquisition (down to 140 nm)
Transmitted light detector (ESID or T-PMT); unique transmitted fluorescence Sample Navigation with T-PMT
Spectral detection: > 8 sequential confocal fluorescence channels, up to three parallel confocal fluorescence channels, based on low-noise GaAsP or MA PMTs; adjustable in
increments of 1 nm
Data depth: 8-bit and 16-bit available
Real-time electronics: Microscope, laser, scanning module and additional accessory control; data acquisition and synchronization management through real-time electronics;
oversampling read-out logic for best sensitivity; data transfer between real-time electronics and user PC via LVDS with the ability to evaluate the data
online during image acquisition