Spesifikasi:
Stand: Axio Observer 7, motorized inverted microscope for superresolution microscopy
Z-drive: DC servomotor, opto-electronically coded; smallest Z step 25 nm
XY Piezo scanning stage: motorized; range 130 mm× 100 mm; max sped 100 mm/s;
resolution 0.2 µm; reproducibility: ± 1 µm; absolute accuracy ± 5 µm;
suitable for mounting frames K 160 × 110 mm and Z-Piezo Stage insert
Z-Piezo stage insert: for XY scanning stage, max travel range 500 µm;
smallest Z step size 5 nm, sample holders available for standard 3"×1" slides LabTek chambers, multiwell plates and 36 mm glass-bottom dishes;
level-adjustable and universal stage insert available for standard slides, glass-bottom dishes and LabTekTM chambers.
Filter sets reflector turret: Four exchangeable filter sets available for multi-channel Lattice SIM and SMLM;
each filter set with four precisely mounted ACR-coded(1) filter modules for superresolution microscopy on a motorized six-position turret;
two positions in each turret compatible with standard Push & Click filter modules, e.g. for visual sample observation.
Dual filter sets for Duolink optimized for dual color and double dual color applications:
Filter sets are optimized for dual camera applications, maximum sensitivity, minimal cross-talk and reduced autofluorescence.
Filter slider: Manual filter slider with two positions (for emission filters or a Bertrand lens);
fits into camera adapter of the microscope’s side port; emission filters exchangeable for customizing detection conditions.
Laser module for Elyra 7: Laser coupling with polarization-maintaining single mode fiber (no adjustment of laser coupling by users required)
Laser lines: 405 nm (50 mW), 488 nm (100 mW or 500 mW), 561 nm (100 mW or 500 mW), 642 nm (100 mW) or 640 nm (500 mW);
405 laser can be attenuated by up to 100000 fold (used for activation and back-pumping);
high power lasers (500 mW) can be 10 fold attenuated (488, 561, 642)
Localization precision (SMLM): Typically 10 nm – 20 nm lateral, 20 nm – 40 nm axial, given sufficient signal-to-noise & density
Multi-color imaging (SMLM): Detection of up to two different fluorescent labels (simultaneous with Duolink or quasi simultaneously by fast sequential laser switching)
Acquisition speed (SMLM): sCMOS (dSTORM) and widefield mode > 100 frames per second full chip; 200 frames per second (512 × 512 pixels)
Multi-color (Lattice SIM and SIM Apotome mode): Detection of up to four different fluorescent labels (sequential detection) and simultaneous dual color detection with DuoLink
Max. Field of view (Lattice SIM): 80.14 × 80.14 μm², full-frame recording (1280 × 1280 effective px) with Plan-Apochromat 63× / 1.40 Oil DIC*