Spesifikasi:
Definite: Focus Holding focus to compensate axial drift, typical z-position accuracy: 30 nm;
Specified limits of Definite Focus 3: 0.2 × DOF (Depth of field: DOF ≈ λ /NA²).
Incubation: Stage top incubation with safety lock
Duolink for attachment of two cameras of the same type: Allows attachment of two cameras of the same type to the microscope.
Storage PC with 81 TByte storage capacity: Direct streaming of data and parallel processing while streaming of data possible
Filter sets reflector turret: Flexible filter set available for simultaneous multi-channel acquisition
Filter set with four precisely mounted ACR-coded filter modules for super-resolution microscopy on a motorized six-position turret;
Two positions in the turret compatible with standard Push & Click filter modules, e.g., for visual sample observation
Dual filter set for Duolink: Filter sets are optimized for one color (SOLO), dual color (DUO) and four color (QUAD) applications
Filter slider: Manual filter slider with Bertrand lens; fits into the slit below the objective turret
Laser module for Lattice SIM 5: Laser coupling with polarization-maintaining single mode fiber (no adjustment of laser coupling by users required)
Laser lines: 405 nm (50 mW), 488 nm (50 mW), 561 nm (50 MW), 640 nm (50 mW);
405, 488 & 640 nm: diode lasers (DL); 561 nm: frequency doubled diode laser (FDDL);
Direct modulation @ 500:1
Resolution (SIM/ SIM² Apotome): Lateral resolution (XY): down to 320 / 265 nm for 25× (typical experimental FWHM values with sub-resolution beads of 100 nm diameter and excitation at 488 nm)
Multi-color (Lattice SIM and SIM Apotome): Detection of up to four different fluorescent labels (sequential detection) and simultaneous dual-color detection with Duolink
Max. field of view (Lattice SIM) @ ORCA-Fusion BT: 103.21 × 103.21 μm², full-frame recording (1288 × 1288 effective px) with Plan-Apochromat 63× / 1.40 Oil DIC* Max. field of view (SIM Apotome) @ ORCA-Fusion BT: 127 × 127 µm², full frame recording (1288 × 1288 effective px) with Plan-Apochromat 40× / 1.40 Oil;
203.20 × 203.20 µm², full frame recording with LD LCI Plan-Apochromat 25× / 0.8 Imm Corr DIC*;
254 × 254 µm², full frame recording with Plan-Apochromat 20× / 0.8 Air;
651 × 651 µm², full frame recording with EC Plan-Neofluar 10× / 0.3 Air